PUBLICATION
Defining morphologically and genetically distinct GAGBergic/cholinergic amacrine cell subtypes in the vertebrate retina
- Authors
- Li, Y., Yu, S., Jia, X., Qiu, X., He, J.
- ID
- ZDB-PUB-240217-1
- Date
- 2024
- Source
- PLoS Biology 22: e3002506e3002506 (Journal)
- Registered Authors
- He, Jie, Jia, Xinling, Li, Yan, Yu, Shuguang
- Keywords
- none
- MeSH Terms
-
- Amacrine Cells*/metabolism
- Animals
- Cholinergic Agents/metabolism
- Mammals
- Retina/metabolism
- Transcription Factors/metabolism
- Zebrafish*
- PubMed
- 38363811 Full text @ PLoS Biol.
Citation
Li, Y., Yu, S., Jia, X., Qiu, X., He, J. (2024) Defining morphologically and genetically distinct GAGBergic/cholinergic amacrine cell subtypes in the vertebrate retina. PLoS Biology. 22:e3002506e3002506.
Abstract
In mammals, retinal direction selectivity originates from GABAergic/cholinergic amacrine cells (ACs) specifically expressing the sox2 gene. However, the cellular diversity of GABAergic/cholinergic ACs of other vertebrate species remains largely unexplored. Here, we identified 2 morphologically and genetically distinct GABAergic/cholinergic AC types in zebrafish, a previously undescribed bhlhe22+ type and a mammalian counterpart sox2+ type. Notably, while sole sox2 disruption removed sox2+ type, the codisruption of bhlhe22 and bhlhe23 was required to remove bhlhe22+ type. Also, both types significantly differed in dendritic arbors, lamination, and soma position. Furthermore, in vivo two-photon calcium imaging and the behavior assay suggested the direction selectivity of both AC types. Nevertheless, the 2 types showed preferential responses to moving bars of different sizes. Thus, our findings provide new cellular diversity and functional characteristics of GABAergic/cholinergic ACs in the vertebrate retina.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping