PUBLICATION
A novel locomotion-based prepulse inhibition assay in zebrafish larvae
- Authors
- Read, E., Hindges, R.
- ID
- ZDB-PUB-240213-2
- Date
- 2024
- Source
- microPublication. Biology 2024: (Journal)
- Registered Authors
- Hindges, Robert
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 38344062 Full text @ MicroPubl Biol
Citation
Read, E., Hindges, R. (2024) A novel locomotion-based prepulse inhibition assay in zebrafish larvae. microPublication. Biology. 2024:.
Abstract
Sensory gating, measured using prepulse inhibition (PPI), is an endophenotype of neuropsychiatric disorders that can be assessed in larval zebrafish models. However, current PPI assays require high-speed cameras to capture rapid c-bend startle behaviours of the larvae. In this study, we designed and employed a PPI paradigm that uses locomotion as a read-out of zebrafish larval startle responses. PPI percentage was measured at a maximum of 87% and strongly reduced upon administration of the NMDA receptor antagonist, MK-801. This work provides the foundation for simpler and more accessible PPI assays using larval zebrafish to model key endophenotypes of neurodevelopmental disorders.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping