PUBLICATION
Interlaboratory Study on Zebrafish in Toxicology: Systematic Evaluation of the Application of Zebrafish in Toxicology's (SEAZIT's) Evaluation of Developmental Toxicity
- Authors
- Hamm, J.T., Hsieh, J.H., Roberts, G.K., Collins, B., Gorospe, J., Sparrow, B., Walker, N.J., Truong, L., Tanguay, R.L., Dyballa, S., Miñana, R., Schiavone, V., Terriente, J., Weiner, A., Muriana, A., Quevedo, C., Ryan, K.R.
- ID
- ZDB-PUB-240127-9
- Date
- 2024
- Source
- Toxics 12(1): (Journal)
- Registered Authors
- Dyballa, Slyvia, Miñana, Rafael, Muriana, Arantza, Quevedo, Celia, Tanguay, Robyn L., Terriente, Javier
- Keywords
- developmental toxicity, harmonization, high-throughput screening, interlaboratory study, optimization, zebrafish
- MeSH Terms
- none
- PubMed
- 38276729 Full text @ Toxics
Citation
Hamm, J.T., Hsieh, J.H., Roberts, G.K., Collins, B., Gorospe, J., Sparrow, B., Walker, N.J., Truong, L., Tanguay, R.L., Dyballa, S., Miñana, R., Schiavone, V., Terriente, J., Weiner, A., Muriana, A., Quevedo, C., Ryan, K.R. (2024) Interlaboratory Study on Zebrafish in Toxicology: Systematic Evaluation of the Application of Zebrafish in Toxicology's (SEAZIT's) Evaluation of Developmental Toxicity. Toxics. 12(1):.
Abstract
Embryonic zebrafish represent a useful test system to screen substances for their ability to perturb development. The exposure scenarios, endpoints captured, and data analysis vary among the laboratories who conduct screening. A lack of harmonization impedes the comparison of the substance potency and toxicity outcomes across laboratories and may hinder the broader adoption of this model for regulatory use. The Systematic Evaluation of the Application of Zebrafish in Toxicology (SEAZIT) initiative was developed to investigate the sources of variability in toxicity testing. This initiative involved an interlaboratory study to determine whether experimental parameters altered the developmental toxicity of a set of 42 substances (3 tested in duplicate) in three diverse laboratories. An initial dose-range-finding study using in-house protocols was followed by a definitive study using four experimental conditions: chorion-on and chorion-off using both static and static renewal exposures. We observed reasonable agreement across the three laboratories as 33 of 42 test substances (78.6%) had the same activity call. However, the differences in potency seen using variable in-house protocols emphasizes the importance of harmonization of the exposure variables under evaluation in the second phase of this study. The outcome of the Def will facilitate future practical discussions on harmonization within the zebrafish research community.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping