PUBLICATION
An Open-Source Programmable Interface for Sensory-Motor Biotests with Zebrafish (Danio rerio)
- Authors
- Bai, Y., Henry, J., Kreuder, F., Mawdsley, D., Kaslin, J., Wlodkowic, D.
- ID
- ZDB-PUB-240110-14
- Date
- 2024
- Source
- Zebrafish 21(1): 48-52 (Journal)
- Registered Authors
- Kaslin, Jan, Mawdsley, David
- Keywords
- DIY, automation, behavior, biotest, sensory–motor, startle response, zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/physiology
- Larva/physiology
- Perciformes*
- Software
- Zebrafish*/physiology
- PubMed
- 38193768 Full text @ Zebrafish
Citation
Bai, Y., Henry, J., Kreuder, F., Mawdsley, D., Kaslin, J., Wlodkowic, D. (2024) An Open-Source Programmable Interface for Sensory-Motor Biotests with Zebrafish (Danio rerio). Zebrafish. 21(1):48-52.
Abstract
Assessment of animals' sensory-motor functions requires precise and electronically controlled stimuli to induce and quantify specific behavioral phenotypes. However, accessible and inexpensive tools for conducting diverse sensory-motor biotests with fish are lacking. In this work, we present an open-source software and hardware interface that enables automated delivery of three independent and fully programmable stimuli for behavioral bioassays. We demonstrate the proof-of-concept application of this low-cost technology in establishing reproducible fear responses using a mechanical tap-startle stimulus in larval zebrafish. This response is characterized by a sudden burst of motion in response to a nondirectional mechanical stimulus delivered to the fish chamber. We propose that the simplicity and flexibility of this interface offer innovative opportunities for studying sensory-motor functions in various fields, including neurobiology, neuropharmacology, neurotoxicology, and aquatic ecotoxicology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping