PUBLICATION
β-Carboline (FG-7142) modulates fear but not anxiety-like behaviour in zebrafish
- Authors
- Scatterty, K.R., Hamilton, T.J.
- ID
- ZDB-PUB-240107-11
- Date
- 2024
- Source
- Scientific Reports 14: 668668 (Journal)
- Registered Authors
- Hamilton, Trevor
- Keywords
- none
- MeSH Terms
-
- Animals
- Anxiety
- Carbolines/pharmacology
- Drug Inverse Agonism
- Fear
- Humans
- Perciformes*
- Zebrafish*
- PubMed
- 38182703 Full text @ Sci. Rep.
Citation
Scatterty, K.R., Hamilton, T.J. (2024) β-Carboline (FG-7142) modulates fear but not anxiety-like behaviour in zebrafish. Scientific Reports. 14:668668.
Abstract
The β-Carboline FG-7142 is a partial inverse agonist at the benzodiazepine allosteric site on the GABA-A receptor that induces anxiogenic, proconvulsant, and appetite-reducing effects in many species, including humans. Seizure-kindling effects have been well studied, however anxiogenic properties are relatively unexplored. This study aimed to investigate concentration-dependent effects of FG-7142 on anxiety-like behaviour and fear responses in zebrafish (Danio rerio) using the open-field test (OF) and novel object approach test (NOA). A U-shaped distribution was found with maximal responses in increased immobility and reduced distance moved at 10 µM in the NOA but not the OF. Follow up experiments demonstrated a lack of effect in repeated OF testing and no changes in opercular movements. Furthermore, the effect of FG-7142 was reversed with ethanol treatment. These results suggest that FG-7142 elicits a 'freezing' response in zebrafish via the introduction of novelty, suggesting fear-induction. These findings indicate that FG-7142 may act as an agent to promote acute fear responses in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping