PUBLICATION
Hepatic effects of acetochlor chiral isomers in zebrafish and L02 cells
- Authors
- Wang, X., Peng, B., Zhang, C., Wu, M., Xu, W., Cheng, J., Tao, L., Li, Z., Zhang, Y.
- ID
- ZDB-PUB-240105-26
- Date
- 2024
- Source
- The Science of the total environment 913: 169781 (Journal)
- Registered Authors
- Wang, Xin
- Keywords
- Acetochlor, Isomer, L02, Liver, Zebrafish
- MeSH Terms
-
- Animals
- Hepatocytes
- Lipid Metabolism
- Lipids
- Liver*/metabolism
- Toluidines*
- Zebrafish*
- PubMed
- 38176547 Full text @ Sci. Total Environ.
Citation
Wang, X., Peng, B., Zhang, C., Wu, M., Xu, W., Cheng, J., Tao, L., Li, Z., Zhang, Y. (2024) Hepatic effects of acetochlor chiral isomers in zebrafish and L02 cells. The Science of the total environment. 913:169781.
Abstract
The pesticide acetochlor (ACT) is a chiral isomer commonly detected in the global environment, yet its specific impacts on liver function remain poorly understood. We utilized zebrafish and L02 cells as research models to comprehensively investigate how ACT and its chiral isomers affect the liver. Our investigations unveiled that the R, Rac, and S isomers of ACT disrupt hepatic lipid transport, catabolism, and synthesis, leading to delayed yolk sac absorption and the accumulation of lipids in zebrafish embryos. These isomers induce oxidative stress in the liver of zebrafish embryos, reducing antioxidant levels and enzyme activity. The accumulated lipids in the liver render it susceptible to oxidative stress, further exacerbating hepatocyte damage. Hepatocyte damage manifests as extensive vacuolization of liver cells and alterations in liver morphology, which are induced by R, Rac, and S. Furthermore, we elucidated the molecular mechanisms underpinning the disturbance of hepatic lipid metabolism by R, Rac, and S in L02 cells. These compounds stimulate lipid synthesis through the upregulation of the AMPK/SREBP-1c/FAS pathway while inhibiting lipolysis via downregulation of the PPAR-α/CPT-1a pathway. Remarkably, our results highlight that S exhibits significantly higher hepatotoxicity in comparison to R. This study provides valuable insights into the hepatic effects of ACT chiral isomers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping