PUBLICATION
In Situ Hybridization to Characterize Neurulation and Midbrain-Hindbrain Boundary Formation in Zebra Fish
- Authors
- Fuller, J., Dworkin, S.
- ID
- ZDB-PUB-231210-8
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2746: 738573-85 (Chapter)
- Registered Authors
- Dworkin, Seb
- Keywords
- Development, Hindbrain, In situ hybridization, Midbrain, Neurulation, Zebra fish
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Mesencephalon
- Neurulation*
- Rhombencephalon
- Zebrafish*/genetics
- PubMed
- 38070081 Full text @ Meth. Mol. Biol.
Citation
Fuller, J., Dworkin, S. (2024) In Situ Hybridization to Characterize Neurulation and Midbrain-Hindbrain Boundary Formation in Zebra Fish. Methods in molecular biology (Clifton, N.J.). 2746:738573-85.
Abstract
Whole-mount in situ hybridization is cable to harness the inherent advantages of zebra fish as a model organism for developmental biology, particularly when visualizing the formation of the neural tube, specifically at the level of the midbrain-hindbrain boundary. The size and transparency of developing zebra fish embryos allow for the visualization of neural markers in vivo along the length of the developing zebra fish central nervous system. In practice, this technique is useful for examining defects in neurulation and midbrain-hindbrain boundary formation that may arise following gene manipulation, for example, CRISPR mutagenesis. This method describes the process of embryo collection and preparation, RNA probe transcription, probe hybridization in vivo, as well as the process of probe detection and visualization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping