PUBLICATION
Mechanical Ablation of Larval Zebra Fish Spinal Cord
- Authors
- Crossman, S.H., Khabooshan, M.A., Stamatis, S.A., Vandestadt, C., Kaslin, J.
- ID
- ZDB-PUB-231210-7
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2746: 475647-56 (Chapter)
- Registered Authors
- Kaslin, Jan
- Keywords
- Regeneration, Spinal cord injury, Tungsten needle fabrication, Zebra fish
- MeSH Terms
-
- Animals
- Larva
- Mammals
- Nerve Regeneration
- Spinal Cord
- Spinal Cord Injuries*
- Spinal Cord Regeneration*
- Zebrafish
- PubMed
- 38070078 Full text @ Meth. Mol. Biol.
Citation
Crossman, S.H., Khabooshan, M.A., Stamatis, S.A., Vandestadt, C., Kaslin, J. (2024) Mechanical Ablation of Larval Zebra Fish Spinal Cord. Methods in molecular biology (Clifton, N.J.). 2746:475647-56.
Abstract
Unlike mammals, adult and larval zebra fish exhibit robust regeneration following traumatic spinal cord injury. This remarkable regenerative capacity, combined with exquisite imaging capabilities and an abundance of powerful genetic techniques, has established the zebra fish as an important vertebrate model for the study of neural regeneration. Here, we describe a protocol for the complete mechanical ablation of the larval zebra fish spinal cord. With practice, this protocol can be used to reproducibly injure upward of 100 samples per hour, facilitating the high-throughput screening of factors involved in spinal cord regeneration and repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping