PUBLICATION

Knockout of SWS2 in zebrafish (Danio rerio) reveals its roles in feeding and phototactic behaviors

Authors

Wang, Y.Y., Liang, X.F., Lu, K.

ID

ZDB-PUB-231204-9

Date

2023

Source

Gene 897: 148059 (Journal)

Registered Authors

Keywords

SWS2 cone, color, food intake, phototaxis, zebrafish

MeSH Terms

Zebrafish*/genetics
Zebrafish*/metabolism
Opsins*/genetics
Opsins*/metabolism
Phototaxis*
Mammals/metabolism
Retinal Cone Photoreceptor Cells/metabolism
Animals

(all 8)

PubMed

38043833 Full text @ Gene

Abstract

Common ancestor of vertebrates had four cone opsin subfamilies to obtain color vision: ultraviolet-sensitive (SWS1), blue-sensitive (SWS2), middle wavelength sensitive (RH2) and long wavelength sensitive (LWS). Nevertheless, eutherian mammals had lost the SWS2 and RH2 opsins during their nocturnal lifestyle. Many studies had demonstrated the role of SWS1 and LWS cones in feeding, mate choice and skin pigment cell formation. However, the role of SWS2 and RH2 cones remain elusive. In the present study, we used an ideal model visual system, zebrafish, which still have the four cone opsins, to generate a SWS2 knockout zebrafish line. Through various behavioral test, we found that sws2^-/- zebrafish larvae exhibited increased food intake compared with WT. Additionally, there were significantly increased the gene expression of phototransduction pathways in sws2^-/- zebrafish larvae. Compared to WT, mutant zebrafish showed weaker phototaxis of red light and changed sensitivity of yellow, red and blue lights. But both mutant and WT zebrafish preferred the red light than other wavelengths of light. Taken together, we proposed that SWS2 cone is not necessary for feeding and phototaxis in zebrafish.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
cnga3a	AB	standard conditions	Adult	eye	RTPCR	Fig. 4
cnga3a	AB	standard conditions	Day 6	whole organism	RTPCR	Fig. 2
cnga3a	opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Adult	eye	RTPCR	Fig. 4
cnga3a	opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism	RTPCR	Fig. 2
gnat2	AB	standard conditions	Adult	eye	RTPCR	Fig. 4
gnat2	AB	standard conditions	Day 6	whole organism	RTPCR	Fig. 2
gnat2	opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Adult	eye	RTPCR	Fig. 4
gnat2	opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism	RTPCR	Fig. 2
gnb3b	AB	standard conditions	Adult	eye	RTPCR	Fig. 4
gnb3b	AB	standard conditions	Day 6	whole organism	RTPCR	Fig. 2

1 - 10 of 48

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
opn1sw2^{zf3924/zf3924} (AB)	control	Day 6	eating behavior increased process quality, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	control	Adult	detection of visible light process quality, abnormal	Fig. 3
opn1sw2^{zf3924/zf3924} (AB)	control	Adult	phototaxis process quality, abnormal	Fig. 3
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism cnga3a expression increased amount, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism gnat2 expression increased amount, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism gnb3b expression increased amount, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism grk1b expression increased amount, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism grk7a expression increased amount, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism opn1lw1 expression increased amount, abnormal	Fig. 2
opn1sw2^{zf3924/zf3924} (AB)	standard conditions	Day 6	whole organism opn1lw2 expression increased amount, abnormal	Fig. 2

1 - 10 of 19

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
zf3924		Indel	opn1sw2

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
opn1sw2	CRISPR1-opn1sw2	CRISPR
opn1sw2	CRISPR2-opn1sw2	CRISPR

Fish

Fish
AB
opn1sw2^{zf3924/zf3924} (AB)

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Wang et al., 2023 ZDB-PUB-231204-9 PMID:38043833

Knockout of SWS2 in zebrafish (Danio rerio) reveals its roles in feeding and phototactic behaviors

Wang et al., 2023

ZDB-PUB-231204-9
PMID:38043833