PUBLICATION
Differential Developmental Neurotoxicity and Tissue Uptake of the Per- and Polyfluoroalkyl Substance Alternatives, GenX and PFBS
- Authors
- Wasel, O., King, H., Choi, Y.J., Lee, L.S., Freeman, J.L.
- ID
- ZDB-PUB-231110-1
- Date
- 2023
- Source
- Environmental science & technology 57(48): 19274-19284 (Journal)
- Registered Authors
- Freeman, Jennifer
- Keywords
- HFPO−DA, behavior, dopamine, perfluoroalkyl substances, perfluorobutanesulfonic acid, polyfluoroalkyl substances, toxicokinetics, zebrafish
- MeSH Terms
-
- Alkanesulfonic Acids*/metabolism
- Alkanesulfonic Acids*/toxicity
- Animals
- Carbon
- Dopamine
- Fluorocarbons*/toxicity
- Tandem Mass Spectrometry
- Zebrafish
- PubMed
- 37943624 Full text @ Env. Sci. Tech.
Citation
Wasel, O., King, H., Choi, Y.J., Lee, L.S., Freeman, J.L. (2023) Differential Developmental Neurotoxicity and Tissue Uptake of the Per- and Polyfluoroalkyl Substance Alternatives, GenX and PFBS. Environmental science & technology. 57(48):19274-19284.
Abstract
Per- and polyfluoroalkyl substances (PFAS) are a class of synthetic chemicals with several applications. Multiple adverse health effects are reported for longer carbon chain (≤C8) PFAS. Shorter carbon chain PFAS, [e.g., hexafluoropropylene oxide dimer acid (HFPO-DA; GenX) and perfluorobutanesulfonic acid (PFBS)] were introduced as alternatives. Past studies indicate that longer-chain PFAS are neurotoxic targeting the dopamine pathway, but it is not known if shorter-chain PFAS act similarly. This study aimed to evaluate developmental neurotoxicity and tissue uptake of GenX and PFBS using the zebrafish (Danio rerio). First, acute toxicity was assessed by measuring LC50 at 120 h postfertilization (hpf). Body burden was determined after embryonic exposure (1-72 hpf) to sublethal concentrations of GenX or PFBS by LC-ESI-MS/MS. Locomotor activity using a visual motor response assay at 120 hpf and dopamine levels at 72 hpf was assessed after embryonic exposure. PFBS was more acutely toxic and bioaccumulative than GenX. GenX and PFBS caused hyperactivity at 120 hpf, but stronger behavioral alterations were observed for PFBS. An increase in whole organism dopamine occurred at 40 ppb of GenX, while a decrease was observed at 400 ppb of PFBS. Differences detected in dopamine for these two PFAS indicate differential mechanisms of developmental neurotoxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping