PUBLICATION
Quantitation of mitotic cells in the neural tube of zebrafish embryos using automated nuclei counting
- Authors
- Emond, M.R., Jontes, J.D.
- ID
- ZDB-PUB-231002-85
- Date
- 2023
- Source
- STAR protocols 4: 102596102596 (Journal)
- Registered Authors
- Emond, Michelle, Jontes, James
- Keywords
- Cell Biology, Developmental Biology, Neuroscience
- MeSH Terms
-
- Animals
- Cell Nucleus
- Histological Techniques
- Histones
- Neural Tube*
- Zebrafish*
- PubMed
- 37738116 Full text @ STAR Protoc
Citation
Emond, M.R., Jontes, J.D. (2023) Quantitation of mitotic cells in the neural tube of zebrafish embryos using automated nuclei counting. STAR protocols. 4:102596102596.
Abstract
Here, we provide a protocol to automate the quantification of the number of phospho-histone H3-positive cells in the developing nervous system of zebrafish using a custom MATLAB script to identify labeled nuclei. We describe steps for fixation, immunolabeling, and imaging of zebrafish embryos. We then detail the analysis steps using Fiji and MATLAB. This protocol can be used for fixed, immunolabeled tissue, as shown here, or for live samples, such as cells expressing a histone-GFP fusion protein. For complete details on the use and execution of this protocol, please refer to Biswas et al.1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping