PUBLICATION
The conducting state of TRPA1 modulates channel lateral mobility
- Authors
- Sampieri, A., Padilla-Flores, T., Thawani, A.R., Lam, P.Y., Fuchter, M.J., Peterson, R., Vaca, L.
- ID
- ZDB-PUB-231002-196
- Date
- 2023
- Source
- Cell Calcium 116: 102800102800 (Journal)
- Registered Authors
- Lam, Pui Ying, Peterson, Randall
- Keywords
- Allyl isothiocyanate, Cholesterol, Lateral mobility, TRPA1 channel, TRPswitch
- MeSH Terms
-
- Animals
- Cholesterol
- Electrophysiological Phenomena
- TRPA1 Cation Channel
- Transient Receptor Potential Channels*/metabolism
- Zebrafish/metabolism
- PubMed
- 37776645 Full text @ Cell Calcium
Citation
Sampieri, A., Padilla-Flores, T., Thawani, A.R., Lam, P.Y., Fuchter, M.J., Peterson, R., Vaca, L. (2023) The conducting state of TRPA1 modulates channel lateral mobility. Cell Calcium. 116:102800102800.
Abstract
We have studied Danio rerio (Zebrafish) TRPA1 channel using a method that combines single channel electrophysiological and optical recordings to evaluate lateral mobility and channel gating simultaneously in single channels. TRPA1 channel activation by two distinct chemical ligands: allyl isothiocyanate (AITC) and TRPswitch B, results in substantial reduction of channel lateral mobility at the plasma membrane. Incubation with the cholesterol sequestering agent methyl-β-cyclodextrin (MβCD), prevents the reduction on lateral mobility induced by the two chemical agonists. This results strongly suggest that the open conformation of TRPA1 modulates channel lateral mobility probably by facilitating the insertion of the channel into cholesterol-enriched domains at the plasma membrane.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping