PUBLICATION
Loss of dlx5a/dlx6a Locus Alters Non-Canonical Wnt Signaling and Meckel's Cartilage Morphology
- Authors
- Yu, E.P.Y., Saxena, V., Perin, S., Ekker, M.
- ID
- ZDB-PUB-231002-152
- Date
- 2023
- Source
- Biomolecules 13(9): (Journal)
- Registered Authors
- Ekker, Marc, Saxena, Vishal
- Keywords
- Wnt signaling, craniofacial development, dlx, neural crest cells, zebrafish
- MeSH Terms
-
- Animals
- Branchial Region*
- Chondrocytes
- Chondrogenesis
- Wnt Signaling Pathway*
- Zebrafish/genetics
- PubMed
- 37759750 Full text @ Biomolecules
Citation
Yu, E.P.Y., Saxena, V., Perin, S., Ekker, M. (2023) Loss of dlx5a/dlx6a Locus Alters Non-Canonical Wnt Signaling and Meckel's Cartilage Morphology. Biomolecules. 13(9):.
Abstract
The dlx genes encode transcription factors that establish a proximal-distal polarity within neural crest cells to bestow a regional identity during craniofacial development. The expression regions of dlx paralogs are overlapping yet distinct within the zebrafish pharyngeal arches and may also be involved in progressive morphologic changes and organization of chondrocytes of the face. However, how each dlx paralog of dlx1a, dlx2a, dlx5a and dlx6a affects craniofacial development is still largely unknown. We report here that the average lengths of the Meckel's, palatoquadrate and ceratohyal cartilages in different dlx mutants were altered. Mutants for dlx5a-/- and dlx5i6-/-, where the entire dlx5a/dlx6a locus was deleted, have the shortest lengths for all three structures at 5 days post fertilization (dpf). This phenotype was also observed in 14 dpf larvae. Loss of dlx5i6 also resulted in increased proliferation of neural crest cells and expression of chondrogenic markers. Additionally, altered expression and function of non-canonical Wnt signaling were observed in these mutants suggesting a novel interaction between dlx5i6 locus and non-canonical Wnt pathway regulating ventral cartilage morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping