PUBLICATION

Innate colour preference in zebrafish (Danio rerio)

Authors
Hagen, E.V., Zhang, Y., Hamilton, T.J.
ID
ZDB-PUB-230907-65
Date
2023
Source
MethodsX   11: 102342102342 (Journal)
Registered Authors
Hamilton, Trevor
Keywords
Behavioural neuroscience, Behavioural testing, Colour preference testing., Conditioned preference, Fish behaviour, Plus maze, Sex difference
MeSH Terms
none
PubMed
37674864 Full text @ MethodsX
Abstract
Innate (natural) colour preference in animals is used for a variety of behavioural neuroscience purposes in many animal models. In zebrafish, colour preference is often used in combination with place preference testing and some memory tests. However, baseline colour preference seems to differ in the few studies examining this innate behaviour. This necessitates a protocol for reliable colour preference testing to establish preferences prior to using more complex behavioural paradigms. This procedure involves an aquatic plus maze with a central neutral zone and 4 coloured zones: red, green, yellow, blue. Adult zebrafish spent significantly more time in the blue zone compared to the red and yellow zones. There were no sex differences in colour preference. This procedure is a rapid, affordable, straightforward, and effective method to establish baseline colour preference.
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