PUBLICATION
Scalable CRISPR Screens in Zebrafish Using MIC-Drop
- Authors
- Parvez, S., Zhang, T., Peterson, R.T.
- ID
- ZDB-PUB-230906-71
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2707: 319334319-334 (Chapter)
- Registered Authors
- Peterson, Randall
- Keywords
- CRISPR-Cas9, F0 CRISPR screen, Genetic screen, High-throughput, In vivo, Reverse genetics, Zebrafish
- MeSH Terms
-
- Animals
- Cell Culture Techniques*
- Technology
- Zebrafish*/genetics
- PubMed
- 37668922 Full text @ Meth. Mol. Biol.
Citation
Parvez, S., Zhang, T., Peterson, R.T. (2024) Scalable CRISPR Screens in Zebrafish Using MIC-Drop. Methods in molecular biology (Clifton, N.J.). 2707:319334319-334.
Abstract
CRISPR-Cas9 is a powerful tool to interrogate gene function in a targeted and systematic manner. Although the technology has been scaled up for large-scale genetic screens in cell culture, similar scale screens in vivo have been extremely challenging due to the cost, labor, and time required to generate and keep track of thousands of mutant animals. We reported the development of Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform that makes large-scale reverse genetic screens possible in zebrafish. In this chapter, we provide a detailed protocol to conduct large-scale genetic screens using this novel platform.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping