PUBLICATION
Spinal Cord Injury and Assays for Regeneration
- Authors
- Burris, B., Mokalled, M.H.
- ID
- ZDB-PUB-230906-64
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2707: 215222215-222 (Journal)
- Registered Authors
- Mokalled, Mayssa
- Keywords
- Axon tracing, Axonal bridging quantification, Glial bridging quantification, Spinal cord histology, Spinal cord injury, Swim endurance assay
- MeSH Terms
-
- Animals
- Neuroglia
- Spinal Cord Injuries*
- Spinal Cord Regeneration*
- Zebrafish
- PubMed
- 37668915 Full text @ Meth. Mol. Biol.
Citation
Burris, B., Mokalled, M.H. (2024) Spinal Cord Injury and Assays for Regeneration. Methods in molecular biology (Clifton, N.J.). 2707:215222215-222.
Abstract
Due to their renowned regenerative capacity, adult zebrafish are a premier vertebrate model to interrogate mechanisms of innate spinal cord regeneration. Following complete transection to their spinal cord, zebrafish extend glial and axonal bridges across severed tissue, regenerate neurons proximal to the lesion, and regain swim capacity within 8 weeks of injury. Here, we describe methods to perform complete spinal cord transections and to assess functional and cellular recovery during regeneration. For spinal cord injury, a complete transection is performed 4 mm caudal to the brainstem. Swim endurance is quantified as a central readout of functional spinal cord repair. For swim endurance, zebrafish are subjected to a constantly increasing water current velocity until exhaustion, and time at exhaustion is reported. To assess cellular regeneration, histological examination is performed to analyze the extents of glial and axonal bridging across the lesion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping