PUBLICATION
Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish
- Authors
- Jia, X., Wyart, C.
- ID
- ZDB-PUB-230906-58
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2707: 125140125-140 (Chapter)
- Registered Authors
- Wyart, Claire
- Keywords
- Behavioral monitoring, Computer-generated holography, Light patterning, Locomotion, Opsins, Optogenetics, Spatial light modulator, Stable zebrafish transgenic lines
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Larva
- Locomotion
- Neurons
- Optogenetics*
- Zebrafish*/genetics
- PubMed
- 37668909 Full text @ Meth. Mol. Biol.
Citation
Jia, X., Wyart, C. (2024) Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish. Methods in molecular biology (Clifton, N.J.). 2707:125140125-140.
Abstract
Understanding how motor circuits are organized and recruited in order to perform complex behavior is an essential question of neuroscience. Here we present an optogenetic protocol on larval zebrafish that allows spatial selective control of neuronal activity within a genetically defined population. We combine holographic illumination with the use of effective opsin transgenic lines, alongside high-speed behavioral monitoring to dissect the motor circuits of the larval zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping