PUBLICATION
Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ)
- Authors
- Montal, E., Suresh, S., Ma, Y., Tagore, M.M., White, R.M.
- ID
- ZDB-PUB-230906-55
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2707: 839783-97 (Chapter)
- Registered Authors
- White, Richard M.
- Keywords
- CRISPR/Cas9, Cancer modeling, Electroporation, Melanoma, Oncogenes, Somatic transgenesis, Tumor suppressors
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Electroporation
- Electroporation Therapies
- Neoplasms*
- Transgenes
- Zebrafish*/genetics
- PubMed
- 37668906 Full text @ Meth. Mol. Biol.
Citation
Montal, E., Suresh, S., Ma, Y., Tagore, M.M., White, R.M. (2024) Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ). Methods in molecular biology (Clifton, N.J.). 2707:839783-97.
Abstract
Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. We provide a method to model cancer in adult zebrafish in which transgenes can be expressed via electroporation. Using melanoma as an example, we demonstrate the feasibility of expressing oncogenes such as BRAFV600E as well as CRISPR/Cas9 inactivation of tumor suppressors such as PTEN. These approaches can be performed in any genetic background such as existing fluorophore reporter lines or the casper line. These methods can readily be extended to other cell types allowing for rapid adult modeling of cancer in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping