PUBLICATION
Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening
- Authors
- Dasgupta, S., Simonich, M.T., Tanguay, R.L.
- ID
- ZDB-PUB-230906-54
- Date
- 2024
- Source
- Methods in molecular biology (Clifton, N.J.) 2707: 718271-82 (Chapter)
- Registered Authors
- Tanguay, Robyn L.
- Keywords
- Automation, Behavior, Chemicals, Development, Embryos, High-throughput screening, Morphology, Zebrafish
- MeSH Terms
-
- Animals
- Hazardous Substances
- High-Throughput Screening Assays*
- Laboratories
- Policy
- Zebrafish*
- PubMed
- 37668905 Full text @ Meth. Mol. Biol.
Citation
Dasgupta, S., Simonich, M.T., Tanguay, R.L. (2024) Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening. Methods in molecular biology (Clifton, N.J.). 2707:718271-82.
Abstract
Zebrafish-based high-throughput screening has been extensively used to study toxicological profiles of individual chemicals and mixtures, identify novel toxicants, and study modes of action to prioritize chemicals for further testing and policy decisions. Within this chapter, we describe a protocol for automated zebrafish developmental high-throughput screening in our laboratory, with emphasis on exposure setups, morphological and behavioral readouts, and quality control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping