PUBLICATION
Live-Cell Imaging of Endogenous RNA with a Genetically Encoded Fluorogenic Allosteric Aptamer
- Authors
- Peng, Y., Shu, L., Deng, X., Huang, X., Mo, X., Du, F., Tang, Z.
- ID
- ZDB-PUB-230904-55
- Date
- 2023
- Source
- Analytical chemistry 95(37): 13762-13768 (Journal)
- Registered Authors
- Mo, Xianming
- Keywords
- none
- MeSH Terms
-
- Animals
- Aptamers, Nucleotide*/genetics
- Diagnostic Imaging
- Humans
- RNA*/genetics
- RNA, Messenger
- Zebrafish/genetics
- PubMed
- 37661353 Full text @ Anal. Chem.
Citation
Peng, Y., Shu, L., Deng, X., Huang, X., Mo, X., Du, F., Tang, Z. (2023) Live-Cell Imaging of Endogenous RNA with a Genetically Encoded Fluorogenic Allosteric Aptamer. Analytical chemistry. 95(37):13762-13768.
Abstract
Imaging and tracking tools for natural cellular RNA with improved biocompatibility, specificity, and sensitivity are critical to understanding RNA function and providing insights into disease therapeutics. We developed a new genetically encoded sensor using fluorogenic allosteric aptamer (FaApt) for the sensitive imaging of the localization and dynamics of RNA targets in live cells. Target RNAs can be specifically recognized with our sensor by forming perfectly complementary duplexes, which in turn can induce allosteric structural changes of the sensor to refold the native conformation of fluorogenic RNA aptamers. We demonstrated the ability of the sensor to monitor the effect of tumor necrosis factor and small-molecule inhibitor on the expression abundance of CXCL1 and survivin mRNA in human cancer cells, respectively. The asymmetrical distribution of endogenous Squint mRNA was confirmed in developing zebrafish embryos through microinjection of FaApt probes. This study provides an effective molecular tool for sensitive imaging and tracking endogenous RNA in living cells. Due to the high specificity and small size of our sensor system, it is expected to be applied to early diagnosis of RNA marker-related diseases and real-time evaluation of the treatment process.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping