PUBLICATION

adducin 1 is essential for the survival of erythroid precursors via regulating p53 transcription in zebrafish

Authors
Yang, S., Cao, S., Xu, X., Li, Q., Li, J., Guo, J., Wang, F., Bao, Y., Jiang, Z., Zhang, T., Wang, L., Sun, S.
ID
ZDB-PUB-230828-48
Date
2023
Source
iScience   26: 107516107516 (Journal)
Registered Authors
Yang, Shuyan
Keywords
Biochemistry, Biological sciences, Cell biology, Molecular biology
Datasets
GEO:GSE236659
MeSH Terms
none
PubMed
37636049 Full text @ iScience
Abstract
Adducin 1 (Add1) is known as a membrane cytoskeletal protein, but its nuclear function remains unclear. In this study, we generated add1-deficient zebrafish to investigate its role in hematopoiesis. Lack of add1 impaired both primitive and definitive hematopoiesis, preventing healthy erythrocyte development. RNA sequencing revealed activation of the p53 pathway in add1-depleted erythroblast cells, leading to apoptosis at the 14-somites stage and 24 hpf. Interestingly, partial rescue of the anemic phenotype and apoptosis was observed with p53 insufficiency. Mechanistically, ADD1 was found to regulate promoter activity. These findings demonstrate that Add1 plays a crucial role in zebrafish erythropoiesis, involving the p53-mediated apoptotic pathway, expanding its regulatory role beyond cytoskeletal functions.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
bj1GtTransgenic Insertion
la781TgTransgenic Insertion
    y1TgTransgenic Insertion
      zdf1
        Point Mutation
        zf152GtTransgenic Insertion
        zf169TgTransgenic Insertion
          1 - 6 of 6
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          add1MO1-add1MRPHLNO
          tp53MO4-tp53MRPHLNO
          1 - 2 of 2
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          Fish
          No data available
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-add1monoclonal
            IgGRabbit
            1 - 1 of 1
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            1 - 2 of 2
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            Mapping