PUBLICATION

Stability and function of RCL1 are dependent on interaction with BMS1

Authors
Wang, Y., Zhao, Z., Yu, H., Shi, H., Tao, B., He, Y., Chen, J., Peng, J., Gan, M., Lo, L.J.
ID
ZDB-PUB-230716-40
Date
2023
Source
Journal of molecular cell biology   15(7): (Journal)
Registered Authors
Chen, Jun, Li Jan, Lo, Peng, Jinrong
Keywords
BMS1, RCL1, liver development, ribosome biogenesis, ubiquitination, zebrafish
MeSH Terms
  • RNA Precursors/genetics
  • RNA Precursors/metabolism
  • Humans
  • Zebrafish*/genetics
  • Saccharomyces cerevisiae Proteins*/genetics
  • Nuclear Proteins/metabolism
  • RNA, Ribosomal, 18S/genetics
  • RNA, Ribosomal, 18S/metabolism
  • Animals
  • RNA Processing, Post-Transcriptional
(all 10)
PubMed
37451810 Full text @ J. Mol. Cell Biol.
Abstract
During ribosome biogenesis, the small subunit (SSU) processome is responsible for 40S assembly. The BMS1/RCL1 complex is a core component of SSU processome that plays an important role in 18S rRNA processing and maturation. Genetic studies using zebrafish mutants indicate that both Bms1l (Bms1-like) and Rcl1 are essential for digestive organs development. In spite of the vital functions of this complex, the mutual dependence for the stability and function of these two nucleolar proteins remains elusive. In this study, we identified an RCL1-interacting domain in BMS1 conserved in zebrafish and human. Moreover, both protein stability and nucleolar entry of RCL1 depend on BMS1, otherwise leading to RCL1 degradation through the ubiquitination-proteasome pathway. Functional studies reveal that overexpression of RCL1 in BMS1 knockdown cells can partially rescue the defects of 18S rRNA processing and cell proliferation, and hepatocyte-specific overexpression of Rcl1 can resume zebrafish liver development in the bms1l substitution mutant bms1lsq163/sq163but not the knockout mutant bms1lzju1/zju1, attributed to the nucleolar entry of Rcl1 in the former. Our data demonstrate that BMS1 and RCL1 interaction is essential not only for pre-rRNA processing but also for the communication between ribosome biogenesis and cell cycle regulation.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sq163
    		Point Mutation
    zju1
      		Indel
      zju24
        		Indel
        zju602TgTransgenic Insertion
          zju603TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            No data available
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab1-fblmonoclonalIgG1Mouse
            Ab3-bms1polyclonal
              		IgGRabbit
              Ab3-h3polyclonal
                		Rabbit
                Ab4-rcl1polyclonal
                  		IgGRabbit
                  1 - 4 of 4
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                  Orthology
                  No data available
                  Engineered Foreign Genes
                  No data available
                  Mapping
                  No data available
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                  Citation
                  Wang, Y., Zhao, Z., Yu, H., Shi, H., Tao, B., He, Y., Chen, J., Peng, J., Gan, M., Lo, L.J. (2023) Stability and function of RCL1 are dependent on interaction with BMS1. Journal of molecular cell biology. 15(7):.