PUBLICATION
Long-term in toto Imaging of Cellular Behavior During Nerve Injury and Regeneration
- Authors
- Tian, W., González-Suarez, A., Lopez-Schier, H.
- ID
- ZDB-PUB-230616-40
- Date
- 2023
- Source
- Bio-protocol 13: e4665e4665 (Journal)
- Registered Authors
- Lopez-Schier, Hernan
- Keywords
- Glia, Macrophages, Microscopy, Nerve Injury, Repair, Zebrafish
- MeSH Terms
- none
- PubMed
- 37323628 Full text @ Bio Protoc
Citation
Tian, W., González-Suarez, A., Lopez-Schier, H. (2023) Long-term in toto Imaging of Cellular Behavior During Nerve Injury and Regeneration. Bio-protocol. 13:e4665e4665.
Abstract
Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural regenerative capacity, which may be due to neuron-extrinsic mechanisms. Therefore, characterizing the behavior of multiple cells during nerve injury and repair in vivo is a pressing need in regenerative medicine. Here, we detail a method for precise wounding of sensory axons in zebrafish, followed by high-resolution in toto long-term quantitative videomicroscopy of neurons, Schwann cells, and macrophages. This protocol can be easily adapted to study the effects of targeted genetic or metabolic disruptions in zebrafish and other suitable organisms, as well as for screening pharmacological agents with therapeutic potential. Graphical overview.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping