PUBLICATION
Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo
- Authors
- Syahirah, R., Beckman, J., Malik, H., Hsu, A.Y., Deng, Q.
- ID
- ZDB-PUB-230613-32
- Date
- 2023
- Source
- Zebrafish 20(4): 175-179 (Journal)
- Registered Authors
- Deng, Qing, Hsu, Alan, Syahirah Mohd Sabri, Ramizah
- Keywords
- Danio rerio, emergency granulopoiesis, hematopoietic tissue, heterogeneity, neutrophils, timer fluorescence protein
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Light*
- Neutrophils/metabolism
- Zebrafish*/metabolism
- PubMed
- 37306974 Full text @ Zebrafish
Citation
Syahirah, R., Beckman, J., Malik, H., Hsu, A.Y., Deng, Q. (2023) Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo. Zebrafish. 20(4):175-179.
Abstract
Emergency granulopoiesis (EG) is a response to severe inflammation in which increased neutrophils are generated in the hematopoietic tissue. Photolabeling is utilized to distinguish newly developed neutrophils from existing neutrophils. However, this technique requires a strong laser line and labels subsets of the existing neutrophils. Here we create a transgenic zebrafish line that expresses a time-dependent switch from green fluorescent protein (GFP) to red fluorescent protein (RFP) in neutrophils, which allows quantification of EG using simple GFP/RFP ratiometric imaging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping