PUBLICATION
Tools to analyze the organization and formation of the germline cyst in zebrafish oogenesis
- Authors
- Kumar, V., Elkouby, Y.
- ID
- ZDB-PUB-230605-35
- Date
- 2023
- Source
- Development (Cambridge, England) 150(13): (Journal)
- Registered Authors
- Elkouby, Yaniv M.
- Keywords
- 3D Deep-learning data, Germ cell development and morphogenesis, Live manipulation by laser-ablation, Microscopy image analysis, Oogenesis and the Germline Cyst, Zebrafish ovary
- MeSH Terms
-
- Animals
- Female
- Germ Cells/ultrastructure
- Oocytes
- Oogenesis*
- Ovary
- Zebrafish*
- PubMed
- 37272421 Full text @ Development
Citation
Kumar, V., Elkouby, Y. (2023) Tools to analyze the organization and formation of the germline cyst in zebrafish oogenesis. Development (Cambridge, England). 150(13):.
Abstract
Oocytes develop in a cellular organization called the germline cyst, in which germ cells are tightly interconnected and surrounded by somatic cells. The cyst produces oocytes for follicle formation and is a hub for essential processes in meiosis and oocyte differentiation. However, the formation and organization of the cyst, and their contribution to oocyte production in vertebrates remain unclear. Here, we provide tools for three-dimensional and functional in-vivo analyses of the germline cyst in the zebrafish ovary. We describe the use of serial block face - scanning electron microscopy (SBF-SEM) to resolve the three-dimensional architecture of cells and organelles in the cyst at ultrastructure resolution. We present a deep learning-based pipeline for high throughput quantitative analysis of three-dimensional confocal datasets of cysts in vivo. We provide a method for laser-ablation of cellular components for manipulating cyst cells in ovaries. These methods will facilitate the investigation of the cyst cellular organization, expand the toolkit for the study of the zebrafish ovary, and advance our understanding of female developmental reproduction. They can be further applied for the investigation of other developmental systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping