PUBLICATION
SAMPL is a high-throughput solution to study unconstrained vertical behavior in small animals
- Authors
- Zhu, Y., Auer, F., Gelnaw, H., Davis, S.N., Hamling, K.R., May, C.E., Ahamed, H., Ringstad, N., Nagel, K.I., Schoppik, D.
- ID
- ZDB-PUB-230603-30
- Date
- 2023
- Source
- Cell Reports 42: 112573112573 (Journal)
- Registered Authors
- Schoppik, David, Zhu, Yunlu
- Keywords
- CP: Neuroscience, apparatus, balance, behavior, drosophila, high-throughput, locomotion, posture, screening, worm, zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal
- Caenorhabditis elegans*
- Drosophila melanogaster*
- Locomotion
- Posture
- Zebrafish
- PubMed
- 37267107 Full text @ Cell Rep.
Citation
Zhu, Y., Auer, F., Gelnaw, H., Davis, S.N., Hamling, K.R., May, C.E., Ahamed, H., Ringstad, N., Nagel, K.I., Schoppik, D. (2023) SAMPL is a high-throughput solution to study unconstrained vertical behavior in small animals. Cell Reports. 42:112573112573.
Abstract
Balance and movement are impaired in many neurological disorders. Recent advances in behavioral monitoring provide unprecedented access to posture and locomotor kinematics but without the throughput and scalability necessary to screen candidate genes/potential therapeutics. Here, we present a scalable apparatus to measure posture and locomotion (SAMPL). SAMPL includes extensible hardware and open-source software with real-time processing and can acquire data from D. melanogaster, C. elegans, and D. rerio as they move vertically. Using SAMPL, we define how zebrafish balance as they navigate vertically and discover small but systematic variations among kinematic parameters between genetic backgrounds. We demonstrate SAMPL's ability to resolve differences in posture and navigation as a function of effect size and data gathered, providing key data for screens. SAMPL is therefore both a tool to model balance and locomotor disorders and an exemplar of how to scale apparatus to support screens.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping