PUBLICATION
Silver Nanoparticles Cause Neural and Vascular Disruption by Affecting Key Neuroactive Ligand-Receptor Interaction and VEGF Signaling Pathways
- Authors
- Lu, C., Liu, Y., Liu, Y., Kou, G., Chen, Y., Wu, X., Lv, Y., Cai, J., Chen, R., Luo, J., Yang, X.
- ID
- ZDB-PUB-230526-33
- Date
- 2023
- Source
- International Journal of Nanomedicine 18: 269327062693-2706 (Journal)
- Registered Authors
- Liu, Yi, Lu, Chunjiao, Luo, Juanjuan, Wu, Xuewei, Yang, Xiaojun
- Keywords
- developmental toxicity, neurological development, silver nanoparticles, vascular development, zebrafish
- MeSH Terms
-
- Animals
- Humans
- Ligands
- Metal Nanoparticles*/toxicity
- Receptors, Adrenergic, beta-2
- Signal Transduction
- Silver/toxicity
- Vascular Endothelial Growth Factor A/genetics
- Zebrafish*/genetics
- Zebrafish Proteins/genetics
- PubMed
- 37228446 Full text @ Int. J. Nanomedicine
Citation
Lu, C., Liu, Y., Liu, Y., Kou, G., Chen, Y., Wu, X., Lv, Y., Cai, J., Chen, R., Luo, J., Yang, X. (2023) Silver Nanoparticles Cause Neural and Vascular Disruption by Affecting Key Neuroactive Ligand-Receptor Interaction and VEGF Signaling Pathways. International Journal of Nanomedicine. 18:269327062693-2706.
Abstract
Introduction Silver nanoparticles (AgNP) are widely used as coating materials. However, the potential risks of AgNP to human health, especially for neural and vascular systems, are still poorly understood.
Methods The vascular and neurotoxicity of various concentrations of AgNP in zebrafish were examined using fluorescence microscopy. In addition, Illumina high-throughput global transcriptome analysis was performed to explore the transcriptome profiles of zebrafish embryos after exposure to AgNP. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to elucidate the top 3000 differentially expressed genes (DEGs) between AgNP-exposed and control groups.
Results We systematically investigated the neural and vascular developmental toxicities of AgNP exposure in zebrafish. The results demonstrated that AgNP exposure could cause neurodevelopmental anomalies, including a small-eye phenotype, neuronal morphology defects, and inhibition of athletic abilities. In addition, we found that AgNP exposure induces angiogenesis malformation in zebrafish embryos. Further RNA-seq revealed that DEGs were mainly enriched in the neuroactive ligand-receptor interaction and vascular endothelial growth factor (Vegf) signaling pathways in AgNP-treated zebrafish embryos. Specifically, the mRNA levels of the neuroactive ligand-receptor interaction pathway and Vegf signaling pathway-related genes, including si:ch73-55i23.1, nfatc2a, prkcg, si:ch211-132p1.2, lepa, mchr1b, pla2g4aa, rac1b, p2ry6, adrb2, chrnb1, and chrm1b, were significantly regulated in AgNP-treated zebrafish embryos.
Conclusion Our findings indicate that AgNP exposure transcriptionally induces developmental toxicity in neural and vascular development by disturbing neuroactive ligand-receptor interactions and the Vegf signaling pathway in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping