PUBLICATION
Interplay between calcium and sarcomeres directs cardiomyocyte maturation during regeneration
- Authors
- Nguyen, P.D., Gooijers, I., Campostrini, G., Verkerk, A.O., Honkoop, H., Bouwman, M., de Bakker, D.E.M., Koopmans, T., Vink, A., Lamers, G.E.M., Shakked, A., Mars, J., Mulder, A.A., Chocron, S., Bartscherer, K., Tzahor, E., Mummery, C.L., de Boer, T.P., Bellin, M., Bakkers, J.
- ID
- ZDB-PUB-230519-27
- Date
- 2023
- Source
- Science (New York, N.Y.) 380: 758764758-764 (Journal)
- Registered Authors
- Bakkers, Jeroen, Chocron, Sonja
- Keywords
- none
- Datasets
- GEO:GSE224156
- MeSH Terms
-
- Regeneration*
- Zebrafish*/physiology
- Myocytes, Cardiac*/physiology
- Sarcomeres*/physiology
- Animals
- Heart*/physiology
- Calcium*/physiology
- Cell Proliferation
- PubMed
- 37200435 Full text @ Science
Citation
Nguyen, P.D., Gooijers, I., Campostrini, G., Verkerk, A.O., Honkoop, H., Bouwman, M., de Bakker, D.E.M., Koopmans, T., Vink, A., Lamers, G.E.M., Shakked, A., Mars, J., Mulder, A.A., Chocron, S., Bartscherer, K., Tzahor, E., Mummery, C.L., de Boer, T.P., Bellin, M., Bakkers, J. (2023) Interplay between calcium and sarcomeres directs cardiomyocyte maturation during regeneration. Science (New York, N.Y.). 380:758764758-764.
Abstract
Zebrafish hearts can regenerate by replacing damaged tissue with new cardiomyocytes. Although the steps leading up to the proliferation of surviving cardiomyocytes have been extensively studied, little is known about the mechanisms that control proliferation and redifferentiation to a mature state. We found that the cardiac dyad, a structure that regulates calcium handling and excitation-contraction coupling, played a key role in the redifferentiation process. A component of the cardiac dyad called leucine-rich repeat-containing 10 (Lrrc10) acted as a negative regulator of proliferation, prevented cardiomegaly, and induced redifferentiation. We found that its function was conserved in mammalian cardiomyocytes. This study highlights the importance of the underlying mechanisms required for heart regeneration and their application to the generation of fully functional cardiomyocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping