PUBLICATION
A tessellated lymphoid network provides whole-body T cell surveillance in zebrafish
- Authors
- Robertson, T.F., Hou, Y., Schrope, J., Shen, S., Rindy, J., Sauer, J.D., Dinh, H.Q., Huttenlocher, A.
- ID
- ZDB-PUB-230509-40
- Date
- 2023
- Source
- Proceedings of the National Academy of Sciences of the United States of America 120: e2301137120e2301137120 (Journal)
- Registered Authors
- Hou, Yiran, Huttenlocher, Anna
- Keywords
- antigen surveillance, collective cell migration, lymphoid tissue, motility, streaming
- Datasets
- GEO:GSE215189
- MeSH Terms
-
- Animals
- Antigen-Presenting Cells
- Antigens
- Cell Movement
- Lymph Nodes
- Mammals
- Receptors, CCR7
- T-Lymphocytes*
- Zebrafish*
- Zebrafish Proteins
- PubMed
- 37155881 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Robertson, T.F., Hou, Y., Schrope, J., Shen, S., Rindy, J., Sauer, J.D., Dinh, H.Q., Huttenlocher, A. (2023) A tessellated lymphoid network provides whole-body T cell surveillance in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 120:e2301137120e2301137120.
Abstract
Homeostatic trafficking to lymph nodes allows T cells to efficiently survey the host for cognate antigen. Nonmammalian jawed vertebrates lack lymph nodes but maintain diverse T cell pools. Here, we exploit in vivo imaging of transparent zebrafish to investigate how T cells organize and survey for antigen in an animal devoid of lymph nodes. We find that naïve-like T cells in zebrafish organize into a previously undescribed whole-body lymphoid network that supports streaming migration and coordinated trafficking through the host. This network has the cellular hallmarks of a mammalian lymph node, including naïve T cells and CCR7-ligand expressing nonhematopoietic cells, and facilitates rapid collective migration. During infection, T cells transition to a random walk that supports antigen-presenting cell interactions and subsequent activation. Our results reveal that T cells can toggle between collective migration and individual random walks to prioritize either large-scale trafficking or antigen search in situ. This lymphoid network thus facilitates whole-body T cell trafficking and antigen surveillance in the absence of a lymph node system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping