PUBLICATION
Neuroprotective Nanoparticles Targeting the Retina: A Polymeric Platform for Ocular Drug Delivery Applications
- Authors
- Colucci, P., Giannaccini, M., Baggiani, M., Kennedy, B.N., Dente, L., Raffa, V., Gabellini, C.
- ID
- ZDB-PUB-230429-65
- Date
- 2023
- Source
- Pharmaceutics 15(4): (Journal)
- Registered Authors
- Kennedy, Breandan N.
- Keywords
- drug delivery and targeting, nerve growth factor, ocular posterior segment, oxidative stress, peanut agglutinin, polymeric nanoparticles, retinal degeneration, zebrafish
- MeSH Terms
- none
- PubMed
- 37111581 Full text @ Pharmaceutics
Citation
Colucci, P., Giannaccini, M., Baggiani, M., Kennedy, B.N., Dente, L., Raffa, V., Gabellini, C. (2023) Neuroprotective Nanoparticles Targeting the Retina: A Polymeric Platform for Ocular Drug Delivery Applications. Pharmaceutics. 15(4):.
Abstract
Neuroprotective drug delivery to the posterior segment of the eye represents a major challenge to counteract vision loss. This work focuses on the development of a polymer-based nanocarrier, specifically designed for targeting the posterior eye. Polyacrylamide nanoparticles (ANPs) were synthesised and characterised, and their high binding efficiency was exploited to gain both ocular targeting and neuroprotective capabilities, through conjugation with peanut agglutinin (ANP:PNA) and neurotrophin nerve growth factor (ANP:PNA:NGF). The neuroprotective activity of ANP:PNA:NGF was assessed in an oxidative stress-induced retinal degeneration model using the teleost zebrafish. Upon nanoformulation, NGF improved the visual function of zebrafish larvae after the intravitreal injection of hydrogen peroxide, accompanied by a reduction in the number of apoptotic cells in the retina. Additionally, ANP:PNA:NGF counteracted the impairment of visual behaviour in zebrafish larvae exposed to cigarette smoke extract (CSE). Collectively, these data suggest that our polymeric drug delivery system represents a promising strategy for implementing targeted treatment against retinal degeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping