PUBLICATION
Oxidative Stress and Endoplasmic Reticulum Stress Contributes to Arecoline and Its Secondary Metabolites-Induced Dyskinesia in Zebrafish Embryos
- Authors
- Yan, W., Zhang, T., Li, S., Wang, Y., Zhu, L., Cao, Y., Lai, X., Huang, H.
- ID
- ZDB-PUB-230414-48
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(7): (Journal)
- Registered Authors
- Huang, Huizhe
- Keywords
- arecaidine, arecoline, arecoline N-oxide, dyskinesia, endoplasmic reticulum stress, oxidative stress
- MeSH Terms
-
- Alkaloids*/pharmacology
- Animals
- Areca
- Arecoline*/toxicity
- Endoplasmic Reticulum Stress
- Oxidative Stress
- Zebrafish/metabolism
- PubMed
- 37047326 Full text @ Int. J. Mol. Sci.
Citation
Yan, W., Zhang, T., Li, S., Wang, Y., Zhu, L., Cao, Y., Lai, X., Huang, H. (2023) Oxidative Stress and Endoplasmic Reticulum Stress Contributes to Arecoline and Its Secondary Metabolites-Induced Dyskinesia in Zebrafish Embryos. International Journal of Molecular Sciences. 24(7):.
Abstract
Areca nut has been listed as one of the most addictive substances, along with tobacco, alcohol and caffeine. Areca nut contains seven psychoactive alkaloids; however, the effects of these alkaloids on embryonic development and motor behavior are rarely addressed in zebrafish embryo-larvae. Herein, we investigated the effects of exposure to three alkaloids (arecoline and secondary metabolites-arecaidine and arecoline N-oxide) on the developmental parameters, locomotive behavior, oxidative stress and transcriptome of zebrafish embryos. Zebrafish embryos exposed to different concentrations (0, 0.1, 1, 10, 100 and 1000 μM) of arecoline, arecaidine and arecoline N-oxide showed no changes in mortality and hatchability rates, but the malformation rate of zebrafish larvae was significantly increased in a dose-dependent manner and accompanied by changes in body length. Moreover, the swimming activity of zebrafish larvae decreased, which may be due to the increase in reactive oxygen species and the imbalance between oxidation and antioxidation. Meanwhile, transcriptome analysis showed that endoplasmic reticulum stress and the apoptosis p53 signaling pathway were significantly enriched after exposure to arecoline and arecoline N-oxide. However, arecaidine exposure focuses on protein synthesis and transport. These findings provide an important reference for risk assessment and early warning of areca nut alkaloid exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping