PUBLICATION
Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish
- Authors
- Pose-Méndez, S., Schramm, P., Winter, B., Meier, J.C., Ampatzis, K., Köster, R.W.
- ID
- ZDB-PUB-230413-48
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Köster, Reinhard W.
- Keywords
- developmental biology, neuroscience, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cerebellum/physiology
- Neurons
- Purkinje Cells*/physiology
- Zebrafish*/physiology
- PubMed
- 37042514 Full text @ Elife
Citation
Pose-Méndez, S., Schramm, P., Winter, B., Meier, J.C., Ampatzis, K., Köster, R.W. (2023) Lifelong regeneration of cerebellar Purkinje cells after induced cell ablation in zebrafish. eLIFE. 12:.
Abstract
Zebrafish have an impressive capacity to regenerate neurons in the central nervous system. However, regeneration of the principal neuron of the evolutionary conserved cerebellum, the Purkinje cell (PC), is believed to be limited to developmental stages based on invasive lesions. In contrast, non-invasive cell type specific ablation by induced apoptosis closely represents a process of neurodegeneration. We demonstrate that the ablated larval PC population entirely recovers in number, quickly reestablishes electrophysiological properties, and properly integrates into circuits to regulate cerebellum-controlled behavior. PC progenitors are present in larvae and adults, and PC ablation in adult cerebelli results in an impressive PC regeneration of different PC subtypes able to restore behavioral impairments. Interestingly, caudal PCs are more resistant to ablation and regenerate more efficiently, suggesting a rostro-caudal pattern of de- and regeneration properties. These findings demonstrate that the zebrafish cerebellum is able to regenerate functional PCs during all stages of the animal's life.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping