PUBLICATION
The mitochondrial ribosomal protein mRpL4 regulates Notch signaling
- Authors
- Mo, D., Liu, C., Chen, Y., Cheng, X., Shen, J., Zhao, L., Zhang, J.
- ID
- ZDB-PUB-230404-53
- Date
- 2023
- Source
- EMBO reports 24(6): e55764 (Journal)
- Registered Authors
- Cheng, Xinkai, Zhao, Long
- Keywords
- Drosophila, Notch, mitochondrial ribosomal protein L4, wap, zebrafish
- MeSH Terms
-
- Animals
- Drosophila/genetics
- Drosophila Proteins*/genetics
- Drosophila Proteins*/metabolism
- Drosophila melanogaster/genetics
- Drosophila melanogaster/metabolism
- Gene Expression Regulation, Developmental
- Humans
- Receptors, Notch/genetics
- Receptors, Notch/metabolism
- Ribosomal Proteins/genetics
- Ribosomal Proteins/metabolism
- Wings, Animal/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 37009823 Full text @ EMBO Rep.
Citation
Mo, D., Liu, C., Chen, Y., Cheng, X., Shen, J., Zhao, L., Zhang, J. (2023) The mitochondrial ribosomal protein mRpL4 regulates Notch signaling. EMBO reports. 24(6):e55764.
Abstract
Mitochondrial ribosomal proteins (MRPs) assemble as specialized ribosome to synthesize mtDNA-encoded proteins, which are essential for mitochondrial bioenergetic and metabolic processes. MRPs are required for fundamental cellular activities during animal development, but their roles beyond mitochondrial protein translation are poorly understood. Here, we report a conserved role of the mitochondrial ribosomal protein L4 (mRpL4) in Notch signaling. Genetic analyses demonstrate that mRpL4 is required in the Notch signal-receiving cells to permit target gene transcription during Drosophila wing development. We find that mRpL4 physically and genetically interacts with the WD40 repeat protein wap and activates the transcription of Notch signaling targets. We show that human mRpL4 is capable of replacing fly mRpL4 during wing development. Furthermore, knockout of mRpL4 in zebrafish leads to downregulated expression of Notch signaling components. Thus, we have discovered a previously unknown function of mRpL4 during animal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping