PUBLICATION
In vivo DNA methylation editing in zebrafish
- Authors
- Liang, F., Dong, Z., Ye, J., Hu, W., Bhandari, R.K., Mai, K., Wang, X.
- ID
- ZDB-PUB-230323-36
- Date
- 2023
- Source
- Epigenetics 18: 21923262192326 (Journal)
- Registered Authors
- Hu, Wei, Wang, Xuegeng
- Keywords
- CRISPR/dCas9, DNA methylation editing, Dnmt7, Tet2, Zebrafish
- MeSH Terms
-
- CRISPR-Cas Systems*
- Epigenome
- DNA Methylation*
- Animals
- Zebrafish/genetics
- Gene Editing/methods
- PubMed
- 36945831 Full text @ Epigenetics
Citation
Liang, F., Dong, Z., Ye, J., Hu, W., Bhandari, R.K., Mai, K., Wang, X. (2023) In vivo DNA methylation editing in zebrafish. Epigenetics. 18:21923262192326.
Abstract
The CRISPR/dCas9-based epigenome editing technique has driven much attention. Fused with a catalytic domain from Dnmt or Tet protein, the CRISPR/dCas9-DnmtCD or -TetCD systems possess the targeted DNA methylation editing ability and have established a series of in vitro and in vivo disease models. However, no publication has been reported on zebrafish (Danio rerio), an important animal model in biomedicine. The present study demonstrated that CRISPR/dCas9-Dnmt7 and -Tet2 catalytic domain fusions could site-specifically edit genomic DNA methylation in vivo in zebrafish and may serve as an efficient toolkit for DNA methylation editing in the zebrafish model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping