PUBLICATION
Examining behavioural test sensitivity and locomotor proxies of anxiety-like behaviour in zebrafish
- Authors
- Johnson, A., Loh, E., Verbitsky, R., Slessor, J., Franczak, B.C., Schalomon, M., Hamilton, T.J.
- ID
- ZDB-PUB-230308-47
- Date
- 2023
- Source
- Scientific Reports 13: 37683768 (Journal)
- Registered Authors
- Hamilton, Trevor
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Anxiety Agents*
- Anxiety
- Anxiety Disorders
- Behavior Rating Scale
- Zebrafish*
- PubMed
- 36882472 Full text @ Sci. Rep.
Citation
Johnson, A., Loh, E., Verbitsky, R., Slessor, J., Franczak, B.C., Schalomon, M., Hamilton, T.J. (2023) Examining behavioural test sensitivity and locomotor proxies of anxiety-like behaviour in zebrafish. Scientific Reports. 13:37683768.
Abstract
This study assessed the sensitivity of four anxiety-like behaviour paradigms in zebrafish: the novel tank dive test, shoaling test, light/dark test, and the less common shoal with novel object test. A second goal was to measure the extent to which the main effect measures are related to locomotor behaviours to determine whether swimming velocity and freezing (immobility) are indicative of anxiety-like behaviour. Using the well-established anxiolytic, chlordiazepoxide, we found the novel tank dive to be most sensitive followed by the shoaling test. The light/dark test and shoaling plus novel object test were the least sensitive. A principal component analysis and a correlational analysis also showed the locomotor variables, velocity and immobility, did not predict the anxiety-like behaviours across all behaviour tests.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping