PUBLICATION
Generation of a transparent killifish line through multiplex CRISPR/Cas9-mediated gene inactivation
- Authors
- Krug, J., Perner, B., Albertz, C., Mörl, H., Hopfenmüller, V.L., Englert, C.
- ID
- ZDB-PUB-230224-42
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Englert, Christoph, Perner, Birgit
- Keywords
- developmental biology
- MeSH Terms
-
- Aging/genetics
- Animals
- Clustered Regularly Interspaced Short Palindromic Repeats
- Fundulidae*
- Longevity/physiology
- Mammals/genetics
- Zebrafish/genetics
- PubMed
- 36820520 Full text @ Elife
Citation
Krug, J., Perner, B., Albertz, C., Mörl, H., Hopfenmüller, V.L., Englert, C. (2023) Generation of a transparent killifish line through multiplex CRISPR/Cas9-mediated gene inactivation. eLIFE. 12:.
Abstract
Body pigmentation is a limitation for in vivo imaging and thus for the performance of longitudinal studies in biomedicine. A possibility to circumvent this obstacle is the employment of pigmentation mutants, which are used in fish species like zebrafish and medaka. To address the basis of aging, the short-lived African killifish Nothobranchius furzeri has recently been established as a model organism. Despite its short lifespan, N. furzeri shows typical signs of mammalian aging including telomere shortening, accumulation of senescent cells and loss of regenerative capacity. Here, we report the generation of a transparent N. furzeri line by simultaneous inactivation of three key loci responsible for pigmentation. We demonstrate that this stable line, named klara, can serve as a tool for different applications including behavioral experiments and the establishment of a senescence reporter by integration of a fluorophore into the cdkn1a (p21) locus and in vivo microscopy of the resulting line.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping