PUBLICATION
Loss of ndrg2 Function Is Involved in Notch Activation in Neuromast Hair Cell Regeneration in Zebrafish
- Authors
- Wang, X., Gu, X., Wang, C., He, Y., Liu, D., Sun, S., Li, H.
- ID
- ZDB-PUB-230218-32
- Date
- 2023
- Source
- Molecular neurobiology 60(6): 3100-3112 (Journal)
- Registered Authors
- Liu, Dong, Wang, Xin
- Keywords
- Hair cell regeneration, Supporting cell, Zebrafish, ndrg2
- MeSH Terms
-
- Animals
- Hair
- Hair Cells, Auditory/metabolism
- Lateral Line System*/metabolism
- Neomycin/metabolism
- Neomycin/pharmacology
- Signal Transduction
- Zebrafish*/genetics
- PubMed
- 36800156 Full text @ Mol. Neurobiol.
Citation
Wang, X., Gu, X., Wang, C., He, Y., Liu, D., Sun, S., Li, H. (2023) Loss of ndrg2 Function Is Involved in Notch Activation in Neuromast Hair Cell Regeneration in Zebrafish. Molecular neurobiology. 60(6):3100-3112.
Abstract
The regeneration of hair cells in zebrafish is a complex process involving the precise regulation of multiple signaling pathways, but this complicated regulatory network is not fully understood. Current research has primarily focused on finding molecules and pathways that can regulate hair cell regeneration and restore hair cell functions. Here, we show the role of N-Myc downstream regulated gene 2 (ndrg2) in zebrafish hair cell regeneration. We first found that ndrg2 was dynamically expressed in neuromasts of the developing zebrafish, and this expression was increased after neomycin-induced hair cell damage. Then, ndrg2 loss-of-function larvae showed reduced numbers of regenerated hair cells but increased numbers of supporting cells after neomycin exposure. By in situ hybridization, we further observed that ndrg2 loss of function resulted in the activation of Notch signaling and downregulation of atoh1a during hair cell regeneration in vivo. Additionally, blocking Notch signaling rescued the number of regenerated hair cells in ndrg2-deficient larvae. Together, this study provides evidence for the role of ndrg2 in regulating hair cell regeneration in zebrafish neuromasts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping