PUBLICATION

Author Correction: SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish

Authors
Liang, F., Zhang, Y., Li, L., Yang, Y., Fei, J.F., Liu, Y., Qin, W.
ID
ZDB-PUB-230217-43
Date
2023
Source
Nature communications   14: 879879 (Journal)
Registered Authors
Fei, Jifeng, Liu, Yanmei
Keywords
none
MeSH Terms
none
PubMed
36797310 Full text @ Nat. Commun.
Abstract
The original version of this Article contained an error in the Methods section “Cas9 RNP complex preparation, microinjection and image acquisition in zebrafish”, which incorrectly read ‘One-cell stage zebrafish embryos were injected with 2 nl of a solution containing 5 µM RNP complex or 300 ng/μl Cas9 mRNA and 30 ng/μl gRNA mixture. For SpRY-CBE4max or zSpRY-ABE8e mRNA, 400 ng/μl was used. For the HDR experiment, 300 ng/μl Cas9 mRNA, 30 ng/μl gRNA and 20 ng/μl donor templated were mixed and injected.’ The correct version states ‘One-cell stage zebrafish embryos were injected with 2 nl of a solution containing 5 µM RNP complex or 300 ng/μl Cas9 mRNA and 200 ng/μl gRNA mixture. For SpRY-CBE4max or zSpRY-ABE8e mRNA, 400 ng/μl was used. For the HDR experiment, 300 ng/μl Cas9 mRNA, 200 ng/μl gRNA and 20 ng/μl donor templated were mixed and injected.’ This has been corrected in both the PDF and HTML versions of the Article.
Errata / Notes
This article corrects ZDB-PUB-220616-2.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping