PUBLICATION
SLAMseq resolves the kinetics of maternal and zygotic gene expression during early zebrafish embryogenesis
- Authors
- Bhat, P., Cabrera-Quio, L.E., Herzog, V.A., Fasching, N., Pauli, A., Ameres, S.L.
- ID
- ZDB-PUB-230210-25
- Date
- 2023
- Source
- Cell Reports 42: 112070112070 (Journal)
- Registered Authors
- Pauli, Andrea
- Keywords
- CP: Developmental biology, embryogenesis, maternal-to-zygotic transition, metabolic RNA sequencing, zebrafish, zygotic genome activation
- Datasets
- GEO:GSE185283
- MeSH Terms
-
- Animals
- Embryonic Development*/genetics
- Gene Expression Regulation, Developmental
- RNA, Messenger/metabolism
- Transcriptome/genetics
- Zebrafish*/metabolism
- Zygote/metabolism
- PubMed
- 36757845 Full text @ Cell Rep.
Citation
Bhat, P., Cabrera-Quio, L.E., Herzog, V.A., Fasching, N., Pauli, A., Ameres, S.L. (2023) SLAMseq resolves the kinetics of maternal and zygotic gene expression during early zebrafish embryogenesis. Cell Reports. 42:112070112070.
Abstract
The maternal-to-zygotic transition (MZT) is a key developmental process in metazoan embryos that involves the activation of zygotic transcription (ZGA) and degradation of maternal transcripts. We employed metabolic mRNA sequencing (SLAMseq) to deconvolute the compound embryonic transcriptome in zebrafish. While mitochondrial zygotic transcripts prevail prior to MZT, we uncover the spurious transcription of hundreds of short and intron-poor genes as early as the 2-cell stage. Upon ZGA, most zygotic transcripts originate from thousands of maternal-zygotic (MZ) genes that are transcribed at rates comparable to those of hundreds of purely zygotic genes and replenish maternal mRNAs at distinct timescales. Rapid replacement of MZ transcripts involves transcript decay features unrelated to major maternal degradation pathways and promotes de novo synthesis of the core gene expression machinery by increasing poly(A)-tail length and translation efficiency. SLAMseq hence provides insights into the timescales, molecular features, and regulation of MZT during zebrafish embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping