PUBLICATION
Passer, a highly active transposon from a fish genome, as a potential new robust genetic manipulation tool
- Authors
- Wang, S., Gao, B., Miskey, C., Guan, Z., Sang, Y., Chen, C., Wang, X., Ivics, Z., Song, C.
- ID
- ZDB-PUB-230124-6
- Date
- 2023
- Source
- Nucleic acids research 51(4): 1843-1858 (Journal)
- Registered Authors
- Gao, Bo
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Transposable Elements*
- Fishes*/genetics
- Gene Transfer Techniques
- Genetic Techniques*
- Humans
- Transposases/genetics
- PubMed
- 36688327 Full text @ Nucleic Acids Res.
Citation
Wang, S., Gao, B., Miskey, C., Guan, Z., Sang, Y., Chen, C., Wang, X., Ivics, Z., Song, C. (2023) Passer, a highly active transposon from a fish genome, as a potential new robust genetic manipulation tool. Nucleic acids research. 51(4):1843-1858.
Abstract
The discovery of new, active DNA transposons can expand the range of genetic tools and provide more options for genomic manipulation. In this study, a bioinformatics analysis suggested that Passer (PS) transposons, which are members of the pogo superfamily, show signs of recent and current activity in animals and may be active in some species. Cell-based transposition assays revealed that the native PS transposases from Gasterosteus aculeatus and Danio rerio displayed very high activity in human cells relative to the Sleeping Beauty transposon. A typical overproduction inhibition phenomenon was observed for PS, and transposition capacity was decreased by ∼12% with each kilobase increase in the insertion size. Furthermore, PS exhibited a pronounced integration preference for genes and their transcriptional regulatory regions. We further show that two domesticated human proteins derived from PS transposases have lost their transposition activity. Overall, PS may represent an alternative with a potentially efficient genetic manipulation tool for transgenesis and mutagenesis applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping