PUBLICATION
Gata2-regulated Gfi1b expression controls endothelial programming during endothelial-to-hematopoietic transition
- Authors
- Koyunlar, C., Gioacchino, E., Vadgama, D., de Looper, H.W.J., Zink, J., Ter Borg, M., Hoogenboezem, R., Havermans, M., Sanders, M.A., Bindels, E., Dzierzak, E.A., Touw, I.P., de Pater, E.
- ID
- ZDB-PUB-230118-9
- Date
- 2023
- Source
- Blood advances 7(10): 2082-2093 (Journal)
- Registered Authors
- de Looper, Hans, de Pater, Emma
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation
- Female
- GATA2 Deficiency*
- GATA2 Transcription Factor/genetics
- GATA2 Transcription Factor/metabolism
- Hematopoietic Stem Cells/metabolism
- Mice
- Pregnancy
- Proto-Oncogene Proteins/genetics
- Repressor Proteins/genetics
- Transcription Factors/genetics
- Zebrafish*/metabolism
- PubMed
- 36649572 Full text @ Blood Adv
Citation
Koyunlar, C., Gioacchino, E., Vadgama, D., de Looper, H.W.J., Zink, J., Ter Borg, M., Hoogenboezem, R., Havermans, M., Sanders, M.A., Bindels, E., Dzierzak, E.A., Touw, I.P., de Pater, E. (2023) Gata2-regulated Gfi1b expression controls endothelial programming during endothelial-to-hematopoietic transition. Blood advances. 7(10):2082-2093.
Abstract
The first hematopoietic stem cells (HSCs) are formed through endothelial-to-hematopoietic transition (EHT) events during embryonic development. The transcription factor GATA2 is a crucial regulator of EHT and HSC function throughout life. Because GATA2 haploinsufficiency patients have inborn mutations, prenatal defects are likely to have an influence on disease development. In mice, Gata2 haploinsufficiency (Gata2+/-) reduces the number and the functionality of embryonic hematopoietic stem and progenitor cells (HSPCs) generated through EHT. However, the embryonic HSPC pool is heterogeneous and the mechanisms underlying this defect in Gata2+/- embryos are unclear. Here, we investigated whether Gata2 haploinsufficiency selectively affects a cellular subset undergoing EHT. We show that Gata2+/- HSPCs initiate but cannot fully activate hematopoietic programming during EHT. In addition, due to reduced activity of the endothelial repressor Gfi1b, Gata2+/- HSPCs cannot repress the endothelial identity to complete maturation. Finally, we show that hematopoietic-specific induction of gfi1b can restore HSC production in gata2b-null (gata2b-/-) zebrafish embryos. This study illustrates pivotal roles of Gata2 on the regulation of transcriptional network governing HSPC identity throughout EHT.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping