PUBLICATION
Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2
- Authors
- Liu, X., Yu, T., Tan, X., Jin, D., Yang, W., Zhang, J., Dai, L., He, Z., Li, D., Zhang, Y., Liao, S., Zhao, J., Zhong, T.P., Liu, C.
- ID
- ZDB-PUB-230117-8
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Jin, Daqing, Liu, Chi
- Keywords
- developmental biology, zebrafish
- Datasets
- GEO:GSE191068, GEO:GSE183382
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Dinoprostone*
- Kidney/physiology
- Nephrons
- Zebrafish*
- PubMed
- 36645741 Full text @ Elife
Citation
Liu, X., Yu, T., Tan, X., Jin, D., Yang, W., Zhang, J., Dai, L., He, Z., Li, D., Zhang, Y., Liao, S., Zhao, J., Zhong, T.P., Liu, C. (2023) Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2. eLIFE. 12:.
Abstract
In organ regeneration, progenitor and stem cells reside in their native microenvironment, which provides dynamic physical and chemical cues essential to their survival, proliferation, and differentiation. However, the types of cells that form the native microenvironment for renal progenitor cells (RPCs) have not been clarified. Here, single-cell sequencing of zebrafish kidney reveals fabp10a as a principal marker of renal interstitial cells (RICs), which can be specifically labeled by GFP under the control of fabp10a promoter in the fabp10a:GFP transgenic zebrafish. During nephron regeneration, the formation of nephrons is supported by RICs that form a network to wrap the RPC aggregates. RICs that are in close contact with RPC aggregates express Cyclooxygenase 2 (Cox2) and secrete prostaglandin E2 (PGE2). Inhibiting PGE2 production prevents nephrogenesis by reducing the proliferation of RPCs. PGE2 cooperates with Wnt4a to promote nephron maturation by regulating β-catenin stability of RPC aggregates. Overall, these findings indicate that RICs provide a necessary microenvironment for rapid nephrogenesis during nephron regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping