PUBLICATION
Biomineralized Nanoscavenger Abrogates Proinflammatory Macrophage Polarization and Induces Neutrophil Clearance through Reverse Migration during Gouty Arthritis
- Authors
- Mohapatra, A., Rajendrakumar, S.K., Chandrasekaran, G., Revuri, V., Sathiyamoorthy, P., Lee, Y.K., Lee, J.H., Choi, S.Y., Park, I.K.
- ID
- ZDB-PUB-230117-11
- Date
- 2023
- Source
- ACS applied materials & interfaces 15(3): 3812-3825 (Journal)
- Registered Authors
- Choi, Seok-Yong
- Keywords
- gout, macrophage polarization: Inflammation, nanoscavenger, neutrophil reverse migration
- MeSH Terms
-
- Animals
- Arthritis, Gouty*/chemically induced
- Arthritis, Gouty*/drug therapy
- Cyclooxygenase 2
- Cytokines
- Disease Models, Animal
- Inflammation/chemically induced
- Inflammation/drug therapy
- Macrophages
- Mice
- Neutrophils
- Reactive Oxygen Species/adverse effects
- Uric Acid
- Zebrafish
- PubMed
- 36646643 Full text @ ACS Appl. Mater. Interfaces
Citation
Mohapatra, A., Rajendrakumar, S.K., Chandrasekaran, G., Revuri, V., Sathiyamoorthy, P., Lee, Y.K., Lee, J.H., Choi, S.Y., Park, I.K. (2023) Biomineralized Nanoscavenger Abrogates Proinflammatory Macrophage Polarization and Induces Neutrophil Clearance through Reverse Migration during Gouty Arthritis. ACS applied materials & interfaces. 15(3):3812-3825.
Abstract
The deposition of monosodium urate (MSU) crystals induces the overexpression of reactive oxygen species (ROS) and proinflammatory cytokines in residential macrophages, further promoting the infiltration of inflammatory leukocytes in the joints of gouty arthritis. Herein, a peroxidase-mimicking nanoscavenger was developed by forming manganese dioxide over albumin nanoparticles loaded with an anti-inflammatory drug, indomethacin (BIM), to block the secretion of ROS and COX2-induced proinflammatory cytokines in the MSU-induced gouty arthritis model. In the MSU-induced arthritis mouse model, the BIM nanoparticles alleviated joint swelling, which is attributed to the abrogation of ROS and inflammatory cytokine secretions from proinflammatory macrophages that induces neutrophil infiltration and fluid building up in the inflammation site. Further, the BIM nanoparticle treatment reduced the influx of macrophages and neutrophils in the injured region by blocking migration and inducing reverse migration in the zebrafish larva tail amputation model as well as in MSU-induced peritonitis and air pouch mouse models. Overall, the current strategy of employing biomineralized nanoscavengers for arthritis demonstrates clinical significance in dual blocking of peroxides and COX2 to prevent influx of inflammatory cells into the sites of inflammation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping