PUBLICATION
A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti-Pseudomonas aeruginosa Infection in Zebrafish
- Authors
- Chen, Y.C., Qiu, W., Zhang, W., Zhang, J., Chen, R., Chen, F., Wang, K.J.
- ID
- ZDB-PUB-230109-13
- Date
- 2022
- Source
- International Journal of Molecular Sciences 24(1): (Journal)
- Registered Authors
- Keywords
- Pseudomonas aeruginosa, Sp-LECin, antibacterial agent, antimicrobial peptide, membrane permeability
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents/chemistry
- Anti-Bacterial Agents/pharmacology
- Anti-Infective Agents*/pharmacology
- Antimicrobial Cationic Peptides/chemistry
- Antimicrobial Peptides
- Biofilms
- Microbial Sensitivity Tests
- Pseudomonas Infections*/drug therapy
- Pseudomonas aeruginosa/metabolism
- Zebrafish/metabolism
- PubMed
- 36613722 Full text @ Int. J. Mol. Sci.
Citation
Chen, Y.C., Qiu, W., Zhang, W., Zhang, J., Chen, R., Chen, F., Wang, K.J. (2022) A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti-Pseudomonas aeruginosa Infection in Zebrafish. International Journal of Molecular Sciences. 24(1):.
Abstract
New antimicrobial agents are urgently needed to address the increasing emergence and dissemination of multidrug-resistant bacteria. In the study, a chemically synthesized truncated peptide containing 22-amino acids derived from a C-type lectin homolog SpCTL6 of Scylla paramamosain was screened and found to exhibit broad-spectrum antimicrobial activity, indicating that it is an antimicrobial peptide (AMP), named Sp-LECin. Sp-LECin possessed the basic characteristics of most cationic AMPs, such as positive charge (+4) and a relatively high hydrophobicity (45%). After treatment with Sp-LECin, the disruption of microbial membrane integrity and even leakage of cellular contents was observed by scanning electron microscopy (SEM). In addition, Sp-LECin could bind lipopolysaccharide (LPS), increase the outer and inner membrane permeability and induce reactive oxygen species (ROS) production, ultimately leading to the death of Pseudomonas aeruginosa. Furthermore, Sp-LECin exhibited potent anti-biofilm activity against P. aeruginosa during both biofilm formation and maturation. Notably, Sp-LECin had no obvious cytotoxicity and could greatly improve the survival of P. aeruginosa-infected zebrafish, by approximately 40% over the control group after 72 h of treatment. This study indicated that Sp-LECin is a promising antibacterial agent with the potential to be used against devastating global pathogen infections such as P. aeruginosa.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping