PUBLICATION
A gelatin-based feed for precise and non-invasive drug delivery to adult zebrafish
- Authors
- Ochocki, A.J., Kenney, J.W.
- ID
- ZDB-PUB-230107-2
- Date
- 2023
- Source
- The Journal of experimental biology 226(2): (Journal)
- Registered Authors
- Kenney, Justin W.
- Keywords
- Behavior, Drug delivery, Pharmacology, Sex differences, Strain, Zebrafish
- MeSH Terms
-
- Animals
- Anxiety
- Behavior, Animal
- Dizocilpine Maleate*
- Gelatin
- Male
- Zebrafish*
- PubMed
- 36606734 Full text @ J. Exp. Biol.
Citation
Ochocki, A.J., Kenney, J.W. (2023) A gelatin-based feed for precise and non-invasive drug delivery to adult zebrafish. The Journal of experimental biology. 226(2):.
Abstract
Although the use of adult zebrafish as a model organism has increased in recent years, there is room to refine methods, like drug delivery, to make them less invasive and more precise. Here, we describe the development of a non-invasive gelatin-based feed method that is tailored to animals based on their weight. The feed was readily eaten by zebrafish (< 1 minute) with minimal leaching of compound when placed in water (< 5% in 5 minutes). As a proof-of-principle, we fed fish a NMDAR antagonist (MK-801, 4 mg/kg) prior to the novel tank test. We found that MK-801 caused a general decrease in predator-avoidance/anxiety-like behavior (bottom dwelling) and an increase in locomotion in male fish, but not females. Our simple, easy to prepare, and individually tailored gelatin-based feed enables precisely dosed, non-invasive drug delivery to adult stage zebrafish for the first time.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping