PUBLICATION
Juvenile zebrafish (Danio rerio) are able to recover from lordosis
- Authors
- Printzi, A., Mazurais, D., Witten, P.E., Madec, L., Gonzalez, A.A., Mialhe, X., Zambonino-Infante, J.L., Koumoundouros, G.
- ID
- ZDB-PUB-221216-8
- Date
- 2022
- Source
- Scientific Reports 12: 2153321533 (Journal)
- Registered Authors
- Koumoundouros, Giorgos, Witten, P. Eckhard
- Keywords
- none
- MeSH Terms
-
- Animals
- Bone Remodeling
- Lordosis*/genetics
- Swimming
- Zebrafish*/genetics
- PubMed
- 36513797 Full text @ Sci. Rep.
Citation
Printzi, A., Mazurais, D., Witten, P.E., Madec, L., Gonzalez, A.A., Mialhe, X., Zambonino-Infante, J.L., Koumoundouros, G. (2022) Juvenile zebrafish (Danio rerio) are able to recover from lordosis. Scientific Reports. 12:2153321533.
Abstract
Haemal lordosis, a frequent skeletal deformity in teleost fish, has long been correlated with increased mechanical loads induced by swimming activity. In the present study, we examine whether juvenile zebrafish can recover from haemal lordosis and explore the musculoskeletal mechanisms involved. Juveniles were subjected to a swimming challenge test (SCT) that induced severe haemal lordosis in 49% of the animals and then immediately transferred them to 0.0 total body lengths (TL) per second of water velocity for a week. The recovery from lordosis was examined by means of whole mount staining, histology and gene expression analysis. Results demonstrate that 80% of the lordotic zebrafish are capable of internal and external recovery within a week after the SCT. Recovered individuals presented normal shape of the vertebral centra, maintaining though distorted internal tissue organization. Through the transcriptomic analysis of the affected haemal regions, several processes related to chromosome organization, DNA replication, circadian clock and transcription regulation were enriched within genes significantly regulated behind this musculoskeletal recovery procedure. Genes especially involved in adipogenesis, bone remodeling and muscular regeneration were regulated. A remodeling tissue-repair hypothesis behind haemal lordosis recovery is raised. Limitations and future possibilities for zebrafish as a model organism to clarify mechanically driven musculoskeletal changes are discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping