PUBLICATION
Effect of gadopentetate dimeglumine on bone growth in zebrafish caudal fins
- Authors
- Tang, R., Chen, Y., He, N., Li, Y., Jin, Z., Chen, K.M., Yan, F.
- ID
- ZDB-PUB-221211-3
- Date
- 2022
- Source
- Toxicology letters 374: 11-18 (Journal)
- Registered Authors
- Chen, Yi, Li, Yan
- Keywords
- bone growth, gadolinium toxicity, gadopentetate dimeglumine, zebrafish
- MeSH Terms
-
- Animals
- Bone Development
- Calcium
- Contrast Media/toxicity
- Female
- Gadolinium DTPA*/toxicity
- Magnetic Resonance Imaging/methods
- Male
- Organometallic Compounds*
- Zebrafish
- PubMed
- 36496117 Full text @ Toxicol. Lett.
Citation
Tang, R., Chen, Y., He, N., Li, Y., Jin, Z., Chen, K.M., Yan, F. (2022) Effect of gadopentetate dimeglumine on bone growth in zebrafish caudal fins. Toxicology letters. 374:11-18.
Abstract
Compared with MR plain scanning, gadolinium (Gd)-enhanced MR scanning can provide more diagnostic information. Gadopentetate dimeglumine is generally used as an MR enhancement contrast agent in some countries. It is a member of linear Gd-based contrast agents (GBCAs) which are considered more likely to release free Gd ions (Gd3+) than macrocyclic GBCAs. Gd3+ is one of the most effective known calcium antagonists, and can compete with calcium ions (Ca2+) in Ca2+-related biological reactions. In this study, animal models of tissue regeneration were established by cutting the caudal fins of zebrafish, and the models were exposed with gadopentetate dimeglumine solution for different immersion times of 1, 3, and 5minutes. Three GBCA exposures per week were performed in the first 3 weeks of the follow-up time. Morphological parameters such as regenerative area (RA), bone density, bone thickness and regenerative bone volume (RBV) were quantified using a camera and synchrotron radiation micro CT. RA decreased as total Gd intake increased in both the female group (ρ = -0.784, P < 0.0001) and the male group (ρ = -0.471, P = 0.011). The bone density of the regenerated bone increased after Gd exposure in the treated groups. The morphology of the regenerated bone from the treated groups became shorter and thicker. Our results showed that gadopentetate dimeglumine had osteogenic toxicity in zebrafish. DATA AVAILABILITY STATEMENT: The data from this study are available from the corresponding author upon reasonable request.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping