PUBLICATION

ftr82 is necessary for hair cell morphogenesis and auditory function during zebrafish development

Authors
Yin, G., Qian, F., Yao, J., Wang, Z., Wang, X., Liu, D., Wang, C.
ID
ZDB-PUB-221205-2
Date
2022
Source
Journal of genetics and genomics = Yi chuan xue bao   50(2): 77-86 (Journal)
Registered Authors
Liu, Dong, Wang, Xin
Keywords
TRIM protein, ftr82, hair cell development, hearing loss, zebrafish
MeSH Terms
  • Animals
  • Deafness*/genetics
  • Embryonic Development
  • Hair Cells, Auditory
  • Hearing
  • Zebrafish*/genetics
PubMed
36464225 Full text @ J. Genet. Genomics
Abstract
Damages of sensory hair cells (HCs) are mainly responsible for sensorineural hearing loss, while the pathological mechanism remains not fully understood due to the many potential deafness genes unidentified. Ftr82, a member of the largely TRIMs family in fish, has been found specifically expressed in the otic vesicle while its function is still unclear. Here, we investigated the roles of ftr82 in HC development and hearing function utilizing the zebrafish model. The results of in situ hybridization illustrated that ftr82 was always restricted to localize in otic vesicles at different stages. The defects of HCs were observed both in ftr82 morphants and mutants, including significantly decreased crista HCs, shortened cilia as well as remarkably reduced functional HCs in neuromasts, which could be successfully rescued by co-injection of exogenous ftr82 mRNA. The behavior assay of startle response indicated that larvae lacking of ftr82 exhibited lower sensitivity to external sound stimuli. Further research revealed that the loss of HCs was mainly caused by cell apoptosis mediated by caspase-3 activation. Our study demonstrates that ftr82 is a crucial hearing-related gene that regulates the HC morphogenesis and auditory function performing, which provides new insight into the rapid identification of the deafness gene.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping