PUBLICATION

Identification and expression analysis of zebrafish gnaq in the hypothalamic-Pituitary-Gonadal axis

Authors
Wang, C., Yang, L., Xiao, T., Li, J., Liu, Q., Xiong, S.
ID
ZDB-PUB-221129-4
Date
2022
Source
Frontiers in genetics   13: 10157961015796 (Journal)
Registered Authors
Keywords
embryonic development, gnaq/Gαq, gonads, reproduction cycle, zebrafish
MeSH Terms
none
PubMed
36437928 Full text @ Front Genet
Abstract
The G proteins have emerged as essential molecular switches in a wide variety of signal transduction pathways. Gαq, encoded by G protein subunit alpha q (gnaq), is a member of the G proteins and participates in regulating important biological activities in mammals; however, its function and regulatory mechanism in teleost remain largely unclear. In the current study, we cloned the cDNA of gnaq from zebrafish (Danio rerio) and investigated the expression characteristics of Gαq/gnaq in reproductive tissues. RT-PCR and WISH analyses showed that gnaq was widely expressed in zebrafish tissues, with high expression in the brain, olfactory brain, and hypothalamus. During the embryonic development stage, the gnaq was mainly distributed in the hypothalamus after 72 h post-fertilization. In addition, immunohistochemistry analysis revealed that the Gαq protein was highly expressed in the diffuse nucleus of the inferior hypothalamic lobe (DIL), ventral zone of the periventricular hypothalamus (Hv), and caudal zone of the periventricular hypothalamus (Hc) in adult zebrafish. Furthermore, in the gonads, the Gαq protein was found in oocytes of all stages, except spermatids. Lastly, the gnaq mRNA exhibited relatively low expression in gonads on Day 4 during the reproductive cycle, while increasing drastically in the hypothalamus and pituitary afterward. Altogether, our results suggest that gnaq/Gαq might be important in fish reproduction.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
Fish
AB
1 - 1 of 1
Show
Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-gnaqRabbit
1 - 1 of 1
Show
Orthology
Gene Orthology
gnaq
1 - 1 of 1
Show
Engineered Foreign Genes
No data available
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Wang, C., Yang, L., Xiao, T., Li, J., Liu, Q., Xiong, S. (2022) Identification and expression analysis of zebrafish gnaq in the hypothalamic-Pituitary-Gonadal axis. Frontiers in genetics. 13:10157961015796.