PUBLICATION
Heterogeneities of zebrafish vasculature development studied by a high throughput light-sheet flow imaging system
- Authors
- Yang, G., Wang, L., Qin, X., Chen, X., Liang, Y., Jin, X., Chen, C., Zhang, W., Pan, W., Li, H.
- ID
- ZDB-PUB-221126-3
- Date
- 2022
- Source
- Biomedical Optics Express 13: 534453575344-5357 (Journal)
- Registered Authors
- Pan, Weijun
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 36425637 Full text @ Biomed. Opt. Express
Citation
Yang, G., Wang, L., Qin, X., Chen, X., Liang, Y., Jin, X., Chen, C., Zhang, W., Pan, W., Li, H. (2022) Heterogeneities of zebrafish vasculature development studied by a high throughput light-sheet flow imaging system. Biomedical Optics Express. 13:534453575344-5357.
Abstract
Zebrafish is one of the ideal model animals to study the structural and functional heterogeneities in development. However, the lack of high throughput 3D imaging techniques has limited studies to only a few samples, despite zebrafish spawning tens of embryos at once. Here, we report a light-sheet flow imaging system (LS-FIS) based on light-sheet illumination and a continuous flow imager. LS-FIS enables whole-larva 3D imaging of tens of samples within half an hour. The high throughput 3D imaging capability of LS-FIS was demonstrated with the developmental study of the zebrafish vasculature from 3 to 9 days post-fertilization. Statistical analysis shows significant variances in trunk vessel development but less in hyaloid vessel development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping